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Black Widow
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This is a very easy boss if you have the shotgun. Simply stand in one spot, aim
for the huge spider, and start shooting. It may get a hit or two in on you, but
don't worry about that. But above everything else, making sure you stay still
and just keep shooting.
There are two baby spiders as well. One will emerge immediately after the
battle. Two shotgun bullets will kill this one. The other will come out a bit
later, and surprise you if you're not expecting it. Kill it. This one is
actually easier done than said.